Portugal began life after Cristiano Ronaldo with a 4-1 defeat to Denmark in Braga.
The loss saw them miss the opportunity to secure the top spot in UEFA Nations League Group A4.
Pierre-Emile Hojbjerg gave Denmark the lead with a penalty just before half-time.
Rasmus Hojlund then netted a brace in the space of eight minutes after the break.
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Ronaldo called time on his international career last week after a record 234 appearances and 146 goals.
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