Thomas Tuchel has left Josh King, James Garner and Jarrad Branthwaite out of England’s matchday squad to face Spain.
England travelled with 26 players, but UEFA rules allow only 23 to be named in the squad for Nations League fixtures.
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The return to fitness of Ezri Konsa and Djed Spence, who both missed Thursday’s 2-1 win over Croatia, has contributed to the trio being left out.
Garner came off the bench against Croatia and had a goal disallowed, while Everton team-mate Branthwaite and Fulham midfielder King were unused substitutes.
King had received his first senior England call-up for the final two Nations League fixtures in Group A3 but will now leave the international window without making his debut.
Fellow first-time call-ups Jobe Bellingham and James Hill have both been included in the squad for the clash with the world champions.
